Abstract: This study investigates the pathological characteristics of Hymenolepis nana infection in hamster small intestine tissues and the expression of Mucin 2（MUC2）. A total of 60 pet hamsters were randomly selected from the Guiyang pet market. Using a saturated salt flotation method, the hamster feces were examined for parasite eggs, and based on the degree of Hymenolepis nana infection, hamsters were categorized into uninfected, asymptomatic, mild infection, and severe infection groups. Hamsters were dissected to collect both the intestinal parasites and the small intestinal tissues. Subsequently, the tissues were sectioned and subjected to staining techniques such as Hematoxylin-Eosin（H.E.）, Alcian Blue-Periodic Acid-Schiff（AB-PAS）, and immunohistochemistry. Real-time quantitative PCR（qPCR） was employed to analyze the relative expression of the MUC2 gene in the hamster small intestine. The results revealed a 74.5% infection rate of Hymenolepis nana in hamsters, which led to pathological alterations including damage to the hamster’s small intestinal villi, degeneration and necrosis of epithelial cells, abnormal goblet cells, and infiltration of leukocyte. In comparison to the uninfected group, the asymptomatic group showed no significant differences in goblet cell count or relative expression levels of the MUC2 gene（P ＞ 0.05）. However, the mild infection group exhibited a highly significant increase in goblet cell count（P＜0.01）, while the severe infection group showed a highly significant decrease（P＜0.01）. Simultaneously, the severe infection group demonstrated a highly significant increase in MUC2 mRNA and MUC2 protein expression levels（P＜0.01）. This indicatesd that Hymenolepis nana infection resultsed in the disruption of the hamster′s small intestinal villi structure, abnormal goblet cell count and morphology, leading to heightened secretion of MUC2 protein. Furthermore, the decrease in goblet cells in the severe infection group are inversely related to the secretion of MUC2 protein.