Abstract: In order to explore the salt tolerance of different varieties（lines） of Leymus chinensis（L. chinensis）, the effects of NaCl stress at different concentrations（200, 400, and 600 mmol/L） on germination and physiological indexes at seedling stage were analyzed in JM007, JM009, Jisheng 1, and Northeast L. chinensis. Using purified water/Hoagland solution as the control（CK）, the salt tolerance coefficient of each single index was analyzed by principal component analysis, relationship analysis and membership function method, and the salt tolerance of L. chinensis was comprehensively evaluated. The results showed that all the indices in germination and seedling stages of L. chinensis were affected by NaCl stress, but the sensitivity of different indices to NaCl stress was different. With the increase of NaCl concentration, the relative germination rate, relative germination potential, relative germination index, relative vitality index, relative embryo length and relative radicle length of seeds decreased gradually. In addition, the inhibition effects of NaCl stress on the germination growth of seedlings was greater than that of radicle length. Compared with CK, the contents of MDA and Pro in four L. chinensis seedlings under different NaCl concentrations were significantly increased（P＜0.05）; the SOD activity of JM007 seedlings increased gradually, and the SOD activity of JM009, Jisheng No.1 and Northeast L. chinensis seedlings increased first and then decreased. Except for Northeast L. chinensis, the POD and CAT activities of the other three L. chinensis seedlings were significantly increased compared with CK（P＜0.05）. Compared with CK, the SS content of JM007 and JM009 seedlings increased gradually, and the SS content of Jisheng 1 and Northeast L. chinensis seedlings showed a steady development first, then a sharp rise and finally a decline. According to the comprehensive membership function value evaluation（D） value, the order of salt tolerance strength was: JM009＞JM007＞ Jisheng No.1 ＞ Northeast L. chinensis at germination stage, and JM007＞ Jisheng No.1 ＞ JM009＞ Northeast L. chinensis at seedling stage. The results indicated that JM009 had a strong salt tolerance at the germination stage, while JM007 had a strong salt tolerance at the seedling stage.