Abstract: In order to obtain a duck-derived Novel goose parvovirus（NGPV） epidemic strain and express the VP2 protein, in the test, liver tissue samples from dead ducks suffering from short-beaked dwarf syndrome were collected; firstly, the virus was isolated from duck embryos by passaging culture; then, erythrocyte agglutination test, PCR detection, determination of the half lethal dose(ELD₅₀) of duck embryos, and pathogenicity test were performed on the virus; finally, the VP2 protein was performed on induced expression, solubility analysis purification, and Western-blot identification. The results showed that a strain of virus was isolated from clinical samples; the death of duck embryos inoculated with this strain was concentrated between the 48th and 72nd hours after inoculation; the dead duck embryos had thickening chorionic villous urothelium, a large number of hemorrhagic spots on the embryo body, and short beaks, which were the typical characteristics of short-beaked dwarf syndrome; and the strain was named as SCNC01 strain. SCNC01 strain was not agglutinative to 1% chicken erythrocytes; the NGPV NS1 gene sequence could be amplified in its genomic DNA, which showed 90.6%-98.3% similarity to the NGPV NS1 gene sequences in GenBank; SCNC01 strain was in the same branch with MK000549, MN415966, JF333590, KC996729, KT598506, MW929338 and other NGPV strains, and this strain was identified as NGPV. The ELD₅₀ of SCNC01 strain was 1×10^-4.5/0.2 mL, which could cause ducklings to show clinical symptoms such as movement disorder, decreased appetite, depression, beak atrophy, tongue outward turning, white scanty feces and even death. The VP2 protein was induced by IPTG to obtain the expression, and the expression form was inclusion body; after purification by His-Tag purification column, a single band with the same size as the target protein appeared; the protein could specifically bind to NDV positive serum with good reactogenicity. The results suggested that in the experiment a strain of duck-derived NGPV was successfully isolated and the VP2 protein with good reactogenicity was obtained.