Abstract: In order to investigate the drug resistance test of Eimeria tenella（E. tenella） Guiyang strain to six anticoccidial drugs, in the test, one hundred and forty-four one-day old Yellow-Feathered laying hens were randomly divided into six administration groups, including diclazuril group（Group D）, toltrazuril group（Group T）, monensin group（Group M）, chlorpheniramine hydrochloride group（Group Y）, decoquinate group（Group G）, and sulfaclozin sodium group（Group H） as well as infection model group（Group C） and blank control group（Group K）, with 18 hens in each group. At 14 days of age, the six dosing groups were each given the drug continuously for 8 d. At 15 days of age, all groups except group K were orally inoculated with 2×10~4 sporulated oocysts of E. tenella Guiyang strain at one time. Clinical symptoms of chicks in each group were observed; weight gain, relative weight gain rate, feed-to-meat ratio, cecum lesion score, number of oocysts per gram of cecum content and survival rate, optimal anticoccidial activity percentage, reduction rate of cecum lesion score, relative oocyst production and anticoccidial index（ACI） were recorded, and the resistance of E. tenella Guiyang strain to six anticoccidial drugs was comprehensively determined. The results showed that 3 d after oral inoculation with sporulated oocysts of E. tenella Guiyang strain, all groups of chicks showed clinical symptoms such as decreased appetite and bloody stools, except group K. The chicks in groups D, G and Y showed slightly better appetite, water intake and mental status than those in group C, and the symptoms were less severe. Group D had the greatest rate of weight gain and relative weight gain, which was significantly higher than that of group C（P＜0.05）, and had the lowest feed-to-meat ratio, which was significantly lower than that of group C（P＜0.05）. The cecum lesion scores of groups D, Y and G were significantly lower than those of groups C and H（P＜0.05）; the number of oocysts per gram of cecum content of groups D, T, M, Y and G was significantly lower than those of groups C and H（P＜0.05）. The survival rate was 90% in group M and 100% in all other groups; ACI＞180 in groups D, G, and Y, with group D being the best（ACI=196.60）. The E. tenella Guiyang strain was non-resistant to diclazuril, mildly resistant to chlorpheniramine hydrochloride and decoxyquinone, moderately resistant to toltrazuril, and completely resistant to sulfachloropyrazine sodium and monensin. The results indicated that in the prevention and treatment of E. tenella in Guiyang area, diclazuril could be used as the first choice of drug, and chlorpheniramine hydrochloride and decoquinate could be used as the alternative drugs（which inhibited the proliferation of coccidia in the body with better effect）; the use of toltrazuril, sulfochloropyrazine sodium, and mornenbacterium should be stopped; and attention should be paid to the rotation, cross, and joint use of drugs, to avoid the emergence of drug resistance.