Abstract: In order to investigate the effects of adding different proportions of whole corn silage to diets on genes related to small intestinal tissue structure and intestinal barrier function in Hezuo pigs, thirty-two healthy weaned Hezuo piglets with similar body weights were randomly divided into four groups with eight piglets in each（half male and half female）. The pigs in the control group were fed a basal diet, and pigs in the 5%, 10% and 15% groups were fed diets with the addition of 5%, 10% and 15% of whole corn silage diets, respectively. After 120 d of feeding, three Hezuo pigs were randomly selected from each group and slaughtered. Tissue samples were taken from various segments of the small intestine, and one part was fixed with 4% paraformaldehyde and stained with hematoxylin-eosin（H.E.） to determine the height of the small intestinal villi and the depth of the crypts, and then the villus height/crypt depth was calculated; and the other part was used for the detection of the relative expression of mRNA of genes related to the small intestinal intestinal barrier function by real-time fluorescence quantitative PCR. The results showed that compared with the control group, the villus height of all segments of the small intestine in the 10% and 15% groups increased; the crypt depth became shallower, and the villus height/crypt depth increased, of which the villus height of all segments of the intestine in the 10% group was significantly higher than that in the control group（P＜0.05）; the crypt depth of the duodenum became significantly shallower（P＜0.05）, and the villus height/crypt depth of the duodenum and the jejunum was significantly higher（P＜0.05）; in the 5%, 10%, 15% group, the relative mRNA expression of physical barrier-related genes（zoster occludin-1 ZO-1, Occludin, junctional adhesion molecule A JAM-A, Claudin-1）, chemical barrier-related genes（β-defensin 1 PBD1, Secretory mucin 2MUC2, intestinal trichome factor TFF3）, and anti-inflammatory factors（interleukin-10 IL-10 and transforming growth factor-β1 TGF-β1） were increased, and the expression of pro-inflammatory factor genes（Toll-like receptor 2 TLR2, tumor necrosis factor-alpha TNF-α, and interleukin-6 IL-6） were decreased. The mRNA relative expression levels of ZO-1, Claudin-1, MUC2, and IL-10 in all segments of the small intestine in the 10% group were significantly higher than those in the control group（P＜0.05）. The relative mRNA expression levels of JAM-A, TFF3, and TGF-β1 in the duodenum and jejunum in the 10% group were significantly higher than those in the control group（P＜0.05）, and the relative mRNA expression level of IL-6 was significantly lower than that in the control group（P＜0.05）. The results indicated that the diet with 10% whole corn silage could better improve the intestinal tissue structure of Hezuo pigs, enhance the intestinal barrier function, and promote intestinal health.