Abstract: In order to analyze the protein expression pattern of the pectoral muscle tissue of mink at different ages, and to investigate the regulatory mechanism of mink growth and development, in the experiment, nine healthy male Silver Blue mink were selected under the same feeding conditions; their pectoral muscle tissues were taken at 45, 90 and 120 days of age; iTRAQ quantitative proteomics technology was used to construct protein expression profiles of Silver Blue mink for screening for significantly differentially expressed proteins; and GO function enrichment, KEGG signaling pathway enrichment, and time-trend clustering analysis were performed. The results showed that a total of 2,922 proteins were identified in the pectoral muscle tissues of Silver Blue mink; among them, 189 were significantly differentially expressed during the growth of mink（multiplicity of difference ≥1.2 or ≤0.83, P＜0.05）; 25 differentially expressed proteins were related to muscle development. The differentially expressed proteins were mainly involved in biological processes such as ribosome biosynthesis, protein targeting to endoplasmic reticulum, translation, actin-mediated cell contraction, myofilament sliding, and regulation of ATPase activity; signaling pathways were enriched such as ribosome, translation, oxidative phosphorylation, energy metabolism, and myocardial contraction; Dynamic analysis of differentially expressed protein expression showed that the expression patterns of Profile 2 and Profile 5 were significantly enriched, and the expression of differentially expressed proteins in Profile 2 pattern was significantly decreased at 90 days of age, which were mainly involved in translation, protein metabolic process and ribosome biosynthesis. Differential expression proteins in the Profile 5 pattern were most highly expressed at 90 days of age, which were related to skeletal muscle system, myocyte development and oxidative phosphorylation. The results suggested that these differentially expressed proteins could be used as candidate proteins related to mink growth and development, and be used for the elucidation of the molecular regulatory mechanism of mink growth and development and for the cultivation of large-scale and high-quality mink breeds.