Abstract: In order to explore the polymorphism of coding sequence（CDS） of TPM₃ gene in Lantang pigs and Yorkshire pigs, 30 healthy Lantang pigs and 35 healthy Yorkshire pigs in the fattening period were selected as test animals, the cDNA sequence of TPM₃ gene from two pig groups was amplified by PCR and the single nucleotide polymorphisms（SNPs） were analyzed by sequencing. The results showed that six SNPS were found in the cDNA sequence of porcine TPM₃ gene, which covered the 5′UTR region and CDS region; one SNP was found in the 5′UTR region of TPM₃ gene, which was single base deletion; and five SNPs（E3-440, E3-452, E4-565, E6-650 and E6-658） were found in CDS region of TPM₃ gene, including three single base transitions, one single base inversion and one single base deletion. Among them, alanine changed to glycine acid at E3-440, glutamic acid changed to glycine acid at E3-452, isoleucine acid changed to valine acid at E4-565, isoleucine acid changed to threonine acid at E6-650, and leucine acid changed to tryptophan acid at E6-658. E3-452 was associated with glutamic acid content, and glutamic acid, as one of the main umami substances affecting pork, had an impact on pork flavor. The results indicated that E3-452 could be used as an SNP site affecting pork umami.