Effect of probiotics on cytokine concentration in Northeast White Goose serum

In order to develop probiotics with immune function, 80 healthy 20-day-old Northeast White Geese goslings with similar weight hatched at the same time were randomly divided into 8 groups, with 10 replicates in each group and 1 gosling in each replicate. Group 1 was the control group, and drinking water was not treated. From 2 to 8 groups, the single bacillus subtilis and compound bacteria preparations Gln, C34, J2 I3 C5, I3 C5, I3 C5 J3, C5 and J2 C5 isolated from the Microbiology Laboratory of The College of Animal Science and Technology, Heilongjiang Bayi Agricultural University were added to the drinking water. All groups were fed with basic diet. The experimental period was 8 weeks. Serum samples were collected from each group at the end of 4 th and 8 th week, and the contents of cytokines interleukin 2（IL-2）, interleukin 6（IL-6）, interleukin 10（IL-10）, tumor necrosis factor（TNF-α）, interferon α（IFN-α） and interferon γ（IFN-γ） in serum of goslings were measured, and the effects of different probiotics on the concentration of cytokines in gosling serum were analyzed. The results showed that at the end of the 4 th week, the content of IL-2 in serum of goslings fed with C5 and J2 C5 bacteria preparations was significantly different from that of the control group（P＜0.05）. The content of TNF-α in serum of goslings fed C34 and I3 C5 J3 bacteria preparation was significantly different from that of control group（P＜0.05）. At the end of the 8 th week, the content of IL-6 in serum of goslings fed with I3 C5 J3 was significantly different from that of the control group（P＜0.05）. The content of IL-10 in serum of goslings fed C34 bacteria preparation group was significantly different from that of control group（P＜0.05）. Except fed Gln group, the contents of TNF-α and IFN-α in serum of goslings fed with other bacteria preparations were significantly different from those of control group（P＜0.05）. The content of IFN-γ in serum of goslings fed with J2 C5 bacteria preparation was significantly different from that of control group（P＜0.05）. The results indicated that probiotics could improve the cytokine concentration and possibly improve the immune function of geese.