Cloning and bioinformatics analysis of the CDS sequence of ELK1 gene in Northeastern Red deer

In order to investigate the sequence information of CDS sequence of ELK1 gene, in the experiment, mRNA reverse transcription and CDS region amplification were used to determine the sequence of CDS sequence of ELK1 gene, which was cloned and constructed into the eukaryotic expression vector. Bioinformatics methods were used to predict and analyze the structure and physicochemical properties of the protein encoded by the gene. The results showed that the CDS sequence of ELK1 gene was 1 323 bp in length（GenBank accession number ON212657） and encoded 440 amino acids with a molecular mass of 46 200.42 u. The nucleic acid sequence was evolutionarily conserved and showed the highest homology with bovine ELK1 gene（97.99%）. The ELK1 polypeptide chain was hydrophilic, without secretory signal peptide structure, and had an isoelectric point of 5.81; the protein was unstable. The secondary structure of ELK1 protein consisted of irregularly coiled, α-helix, extended chain, β-fold, having ETS structural domain, and was located in the nucleus under subcellular localization.