Analysis of liver tissue development characteristics in embryonic stage of Wuliangshan Black-bone chickens

In order to clarify the development characteristics of liver tissue during embryonic period of Wuliangshan Black-bone chickens, three varieties of eggs, including Arbor Acres（AA）, Hy-Line gray layers and Wuliangshan Black-bone chickens, were incubated at the same time. The embryonic weight and liver weight were measured by weighing method, and the relative liver weight of liver weight/embryonic weight was compared among varieties. H.E. staining of paraffin sections and oil red O staining of frozen sections were used to detect lipid deposition in liver; the mRNA expression changes of FAS and ATGL were detected by RT-qPCR. The results showed that the relative liver weights of Wuliangshan Black-bone chickens at E10 and E14 stages were significantly different from those of AA chickens and Hy-Line gray layers（P＜0.05）. Oil red O staining showed that E18 liver tissue had the darkest staining color. The relative expression of FAS and ATGL in Wuliangshan Black-bone chickens was the highest before E18 and the difference was significant（P＜0.05）. The temporal expression of FAS and ATGL in Wuliangshan Black-bone chickens showed that both of them reached the peak at D0 and had significant difference compared with other stages（P＜0.01）, indicating that the lipid metabolism activity of Wuliangshan Black-bone chickens was more active after embryonic stage than that of broiler and laying hens.