Therapeutic effects of Shenling Baizhu powder on ulcerative colitis in mice

In order to explore the therapeutic effects of Shenling Baizhu powder on ulcerative colitis in mice induced by dextran sulfate sodium, 30 female C57BL/6 mice（6-8 weeks old, weighing 18-20 g） were randomly divided into five groups, namely blank control group, model group, and high, medium, and low dose groups, with six mice in each group. In the first week, each group underwent adaptive feeding. In the second week, except for the blank control group, each group was given 3% dextran sulfate sodium aqueous solution for free drinking water for seven days to establish an acute ulcerative colitis model. In the third week, normal drinking water was replaced, and the blank control group and model group were given physiological saline by gavage. The high, medium, and low dose groups of the formula were given 0.2, 0.1, 0.05 mL/10 g of traditional Chinese medicine solution by gavage according to their body weight. Clinical symptoms of mice were observed and recorded; serum was collected from whole blood; mass concentration changes of immunoglobulin G（IgG）, immunoglobulin A（IgA）, interleukin-8（IL-8）, interleukin-10（IL-10）, diamine oxidase（DAO） and D-lactic acid in serum were detected, and pathological sections of colon were made. The results showed that compared with the blank control group, the mass concentrations of IL-8, IgA, IgG, DAO, D-lactate in the serum of the model group were significantly increased（P＜0.01）, while the mass concentration of IL-10 was significantly reduced（P＜0.01）. After administration, the high, medium, and low dose groups of the mice was alleviated to various degrees, and the mass concentrations of IL-8, IgA, IgG, DAO, D-lactate were all reduced to various degrees, while the mass concentration of IL-10 increased. These results indicated that Shenling Baizhu powder had a good therapeutic effect on ulcerative colitis induced by dextran sulfate sodium. Under the conditions of this experiment, the best therapeutic effect was given at 0.2 mL/10 g of body weight.