Anti-inflammatory Effects of Baicalin （InVitro and InVivo）

Baicalin （BA） is commonly used to treat inflammatory diseases and shows anti-inflammatory effects.The present study aimed to evaluate the analgesic and anti-inflammatory activities of BA both in vitro and in vivo.In animal models,acetic acid-induced writhing was used to assess analgesic activity.In addition,a variety of tests including xylene-induced ear edema test,minimum inhibitory concentration （MIC） assays and acetic acid-induced peritoneal capillary hyperpermeability test were used to evaluate anti-inflammatory activity of BA.BA at 0.2 and 0.1 mg·mL^-1 doses expressed analgesic as well as anti-inflammatory activities in mice.In acetic acid induced writhing test,BA applying three times,twice and once a day significantly inhibited the acetic acid-induced writhing response within 15 min by 7.80% （*p＜0.05）,7.50% （**p＜0.01） and 6.25% （**p＜0.01）.In xylene-induced ear edema test,BA at 0.2,0.1 and 0.05 mg·mL^-1 decreased ear edema by 45.50% （**p＜0.01）,15.20% （*p＜0.05） and 9.10% （*p＜0.05）.In acetic acid-induced peritoneal capillary hyperpermeability test,BA exhibited significant inhibition （*p＜0.05 versus control） of inflammation.In MIC assays,the MIC values of baicalin for S.aureus and Escherichia coli were 125 mg·mL^-1,and the MIC values for the control bacteria ATCC25922 and ATCC25923 were 62.5 mg·mL^-1.These findings suggested baicalin might contain analgesic and anti-inflammatory agents which supported its use in traditional medicine.