Dynamic Observation of Cartilage Damage and Serum IL-1β, TNF-α, IL-6 and IL-17 in Post-traumatic Rat Osteoarthritis Model

Lv Liang-yu; Ma Tian-wen; Yu Yue; Jia Li-na; Xu Xin-yu; Zhao Ming-chao; Chen Hong; Sheng Xuan-bo; Li Ting; Xiao Jian-hua; Gao Li

Journal of Northeast Agricultural University(English Edition) > 2022 > 29(3): 59-65.

Lv Liang-yu, Ma Tian-wen, Yu Yue, Jia Li-na, Xu Xin-yu, Zhao Ming-chao, Chen Hong, Sheng Xuan-bo, Li Ting, Xiao Jian-hua, Gao Li. Dynamic Observation of Cartilage Damage and Serum IL-1β, TNF-α, IL-6 and IL-17 in Post-traumatic Rat Osteoarthritis Model[J]. Journal of Northeast Agricultural University(English Edition), 2022, 29(3): 59-65.

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Dynamic Observation of Cartilage Damage and Serum IL-1β, TNF-α, IL-6 and IL-17 in Post-traumatic Rat Osteoarthritis Model

College of Veterinary Medicine, Northeast Agricultural University, Heilongjiang Key Laboratory Animals and Comparative Medicine

Funds:

Supported by the National Key R&

D Program of China (2017YFD0502200)

the Applied Technology Research and Development Plan of Heilongjiang Province (GX18B023)

Xinjiang Uygur Autonomous Region Science and Technology Support Project Plan (2020E0236)

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Abstract

Abstract

The pathogenesis of animal osteoarthritis（OA） is not clear. The aim of this study was to investigate the dynamic changes of cartilage lesions and serum IL-1β, TNF-α, IL-6 and IL-17 inflammatory factors in a post-traumatic osteoarthritis model in rats, and to explore their values in monitoring the progression of osteoarthritis. Forty male SD rats were randomly divided into the control group（n=20） and the model group（n=20）. The OA model was established by anterior cruciate ligament transection combined with partial medial meniscus resection（ACLT+PMMx） in the model group, and sham operation was performed in the control group. On the 0th, 10th, 20th and 30th days after modeling, the degree of joint swelling and the number of white blood cells in the peripheral blood were evaluated, and tibia samples were taken for macroscopic observation score and pathological observation. The serum levels of IL-1β, TNF-α, IL-6 and IL-17 concentration were detected by enzyme-linked immunosorbent assay（ELISA）. The results showed that the cartilage damage in the tibial surface and pathological observations of rats in the model group gradually increased with time, the eye scores increased and increased significantly on the 20th and 30th days（P＜0.01）, and the joints of the rats were swollen. And the swelling was extremely significant on the 30th day（P＜0.01）. Compared with the control group, the number of white blood cells in the peripheral blood of the model group gradually increased, and the increase was extremely significant on the 20th and 30th days（P＜0.01）. The serum IL-17 concentration in the model group tended to increase, and the increase was significant on the 20th day（P＜0.05） and extremely significant on the 30th day（P＜0.01）; the serum TNF-α and IL-1β concentrations in the model group showed an increasing trend, and the increase was extremely significant on the 10th, 20th and 30th days（P＜0.01）; IL-6 concentration in the serum of the model group showed an increasing trend, and it increased significantly on the 30th day（P＜0.05）. The results showed that in the rat model of traumatic OA, the degree of joint damage gradually increased, and the expression of blood inflammatory indexes IL-1β, TNF-α, IL-17 and IL-6 increased. The research provided a basis for prompting and monitoring the timing of OA treatment and the development of the disease in animal clinical.
- osteoarthritis,
- inflammatory factor,
- cartilage injury,
- rat

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References (22)

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