Fine Genetic Mapping of Dwarf Trait in Cucumber(Cucumis sativus L.) Using a RIL Population
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Graphical Abstract
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Abstract
Plant height in cucumber is not only an important trait for breeding,but also one of the model traits suitable for the study of developmental biology.Amplified fragment length polymorphism (AFLP) and simple sequence repeat (SSR) markers techniques were applied for the construction of genetic linkage maps in cucumber.To understand the dwarf trait genetic basis in cucumber,the quantitative trait loci (QTL) were identified using F6 recombinant inbred line populations (RILs) comprising 336 lines from the cross between the two cultivars D0462 (the dwarf) and DN129 (the vine).In total,six SSR markers and 15 AFLP markers were detected on the five linkage groups covering 152.8 cM with a mean marker interval of 7.28 cM.Only one QTL was found to be linked with plant height and the dwarf trait locus remained in the fourth linkage group.The contributory percentage of the single QTL to plant height was 11.39%.
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