Study on Relationship Between Differential Proteins of Bacillus cereus LBR-4 and Its Salt Tolerance Mechanism

Tan Jia-li; Du Chuan-ying; Wang Jian; Ni He-jia; Gao Ji-guo; Li Hai-tao

Journal of Northeast Agricultural University(English Edition) > 2020 > 27(3): 53-62.

Tan Jia-li, Du Chuan-ying, Wang Jian, Ni He-jia, Gao Ji-guo, Li Hai-tao. Study on Relationship Between Differential Proteins of Bacillus cereus LBR-4 and Its Salt Tolerance Mechanism[J]. Journal of Northeast Agricultural University(English Edition), 2020, 27(3): 53-62.

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Study on Relationship Between Differential Proteins of Bacillus cereus LBR-4 and Its Salt Tolerance Mechanism

College of Life Sciences, Northeast Agricultural University

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Supported by Heilongjiang Province National Science Foundation(LH2020C007)

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Abstract

Abstract

In order to explore the salt tolerance mechanism of Bacillus cereus LBR-4 with salinity of 14% NaCl,differential proteomic analysis of the whole protein of LBR-4 strain expressed under 14% NaCl high salinity condition and normalculture condition （1% NaCl） was studied by two-dimensional electrophoresis and mass spectrometry.The isoelectric point of most detected proteins was between pH 4-7 and the molecular weight distribution was 10-70 ku.Compared with the normal culture condition,the expression level of 118 protein spots in the whole protein expression map changed significantly （accounting for 25.2% of the total protein spots）.The expression level of 78 protein spots increased significantly,including 22 new protein spots that appeared under high salt stress.The expression levels of 40 protein spots decreased significantly,including 18 protein spots that disappeared under high salt stress.By mass spectrometry,six distinct differentially expressed protein spotswere dihydroxy acid dehydratase,cell division protein FtsZ,iron sulfur cluster synthesis protein SufD,unknown carboxylase YngE,hypothetical acetaldehyde dehydrogenase DhaS and phenylalanine acid tRNA ligase alpha subunit.It was speculated that under high salt stress,the cells had protective measures and the secretion of intracellular compatible solutes increased.The iron and sulfur clusters involved in various physiological reactions also activated the stressful suf synthesis pathway,and therate of cell division and reproduction was also slowed down and ensured the normal progress of physiological reactions inthe cells.
- Bacillus cereus LBR-4,
- proteomics,
- two-dimensional electrophoresis,
- protein profile,
- salt tolerance mechanism

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References (31)

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