A PCR-Based Method for Detecting Pathogen of Rice False Smut

Rice false smut is a destructive disease that affects rice grain badly. The disease seriously affects the yield and quality of rice in Heilongjiang Province. In this paper, a pair of specific primers was designed to detect the false smut pathogen rapidly and efficiently. The results showed that the pair of primers had strong specificity for false smut pathogen. In addition, the sensitivity of this primer to the genomic DNA of rice false smut pathogen in PCR reaction was 1 pg. By using these primers, the rice false smut pathogen could be detected within 48 h after inoculation, and a PCR reaction system with good specificity and high sensitivity was established.