Abstract: As the working fluid of pulsating heat pipe（PHP） for the power battery heating element, TiO₂ nanofluid was prepared with distilled water and ethanol as base solution to ensure the operating properties of the power battery at low temperature. The experimental study on the heating performance of power batteries in low temperature environment was carried out. The experimental results show that the thermal transport property with ethanol is better than that with distilled water at low temperature. When the volume fraction of nano TiO₂ fluid is 2% with heat pipe filling rate of 50%, the thermal property of PHP is significantly improved with the optimum performance of PHP at low temperature. For the optimal working medium ratio of ethanol-based liquid, the maximum cell capacities of titanium dioxide nanofluid pulsating heat pipe（TiO₂-PHP） under the charge and discharge modes in low temperature environment can reach 62.91 A·h. In the extremely cold environment of-30 ℃, using the designed heat pipe as heating element to heat the electric vehicle power battery, the improvement of 61.56 A·h from the initial discharge failure can be achieved. The designed TiO₂-PHP has efficient heating performance and thermal conductivity.