Abstract: To study the role of sweet potato IbERF in biotic stress, IbERF1 was cloned from high resistance Fusarium wilt variety ‘Eshu11’. Bioinformatics analysis and the expression levels of IbERF1 in Eshu11 were carried out under Fusarium oxysporum f.sp. batatas（Fob） infection, MeJA and ETH treatment. The result of bioinformatics analysis showed that the full length of IbERF1 CDS sequence was 588 bp, which encoded 195 amino acids. It was presumed that IbERF1 encoding an unstable hydrophilic protein, its molecular weight was 21.97 ku, the isoelectric point was 5.11. Different promoter cis-acting elements such as plant hormone response elements Myb, stress response element MYC, MYB, etc. were found in the promoter region of IbERF1. The multiple sequences alignment and phylogenetic tree analysis revealed that IbERF1 protein was closest to InERF1 B-like. The results of subcellular localization prediction and experiment showed that IbERF1 protein was a nucleus localized protein. The prediction of protein secondary structure revealed that IbERF1 protein contained 27.69% of random coil structure, 53.85% of α-helix structure, 12.31% of extended chain structure, and 6.15% of β-turn. The results of real-time quantitative PCR analysis indicated that the expression level of IbERF1 in ‘Eshu11’ significantly increased（P＜0.05） at 2 hours and 4 hours under Fob infection, and the expression level of IbERF1 is significantly increased（P＜0.05） at 2 h, 4 h, 12 h under MeJA and ETH treatment. To sum up, IbERF1 expression could be induced by Fob, MeJA and ETH treatment.