Abstract: In order to screen the SNP and analyze the structure and function of GABRR2 genes in Yorkshire pigs,30 Yorkshire pigs were used as templates to amplify the exons of GABRR2 gene by PCR. The SNPs were screened after sequence, and the sequences were spliced. And then a series of analyses were carried out on the promoter region, coding region and 3 ’UTR region of GABRR2 gene in Yorkshire pigs by using a variety of bioinformatics software. The results showed that there were four synonymous mutations in c615, c660, c798 and c1134 of GABRR2 gene in Yorkshire pigs. The genes, RNGTT, SRSF12 and ANKRD6, were in the linkage region of GABRR2 gene, and the proteins encoded by the gene interacted with each other. There were two core promoters in the 2 000 bp region upstream of the initiation codon which Sp1 in 386～395 and 769～778, CREB-2 in 757～766,NF-1 in 775～784, and a CpG island in the 1593～1752 base region. There were 14 miRNA binding sites in 3’UTR region, of which the scores of miR-365-3 p, miR-497-5 p, miR-5195-3 p and miR-145-5 p were higher than 80.Conclusion: GABRR2 gene had four synonymous mutations in the CDS region, which were highly conserved. It encoded a hydrophilic protein with four transmembrane structures and one signal peptide GABRR2 gene, which may be closely related to the function of RNGTT gene. The two core promoter regions contained four transcription factor binding sites of Sp1, CREB-2 and NF-1, and a characteristic CpG island in promoter regions. There were four miRNA binding regions in the 3’UTR region: miR-365-3 p, miR-497-5 p, miR-5195-3 p and miR-145-5 p.