Abstract: The differential expression patterns of growth and development of IGF-1-PI3 K/AKT pathway in longissimus dorsi muscles of Guangxi Bama mini-pig and Landrace were studied in this paper, providing molecular theoretical basis for future research on the growth and meat quality of Guangxi Bama mini-pig. Porcine INSR, IRS1,IGF-1, PI3 K, PI3 KR1, PDK1, PTEN, AKT1, FoxO1, m TOR, 4 EBP1 and β-actin genes of pig were cloned and the fluorescence quantitative PCR standard curve was constructed. The expression of above genes in the longest longissimus dorsi muscles of Guangxi Bama mini-pigs and Landrace pigs at 1, 30, 60, 90 and 180 days were detected by Real-time PCR. At the same growth and development stage, the expression of PTEN and 4 EBP1 genes was significantly higher than that of other genes in the same pig species, FoxO1, PI3 KR1 and AKT1 genes were moderately expressed, IGF-1, INSR, IRS1, PI3 K and PDK1 genes were lowly expressed. There were significant differences in gene expression at different growth and development stages, and there were differences in gene expression at the same growth and development time between the two breeds. The expression patterns of IGF-1-PI3 K/AKT pathway genes in Guangxi Bama mini-pig and Landrace showed significantly differences in breeds and in space-time, PTEN, FoxO1 and 4 EBP1 gene may be one of the reasons for the differences in meat production and meat quality traits between the two breeds of pigs.