Abstract: In order to screen the key regulatory genes of meat quality traits in black Angus cattle, the longissimus dorsi muscles of 3 24-month-old black Angus cattle and Simmental cattle were used as experimental materials. Illumina HiSeqTMtechnique was used to sequence all samples and bioinformatics methods and related software were used to classify and analyze the sequencing results. The differentially expressed genes between black Angus cattle and Simmental samples were 409, of which 115 were up-regulated and 294 were down-regulated. Further analysis showed that the expression of PPARD, which was related to meat quality traits, was significantly higher in Black Angus than that of Simmental（P＜0.01）. The experimental results were consistent with those of the transcriptional group by RT-qPCR. These results not only showed that the expression of PPARD in black Angus cattle was higher than that in Simmental cattle, but also indicated that the gene had a certain regulatory effect on beef quality. It also has certain reference value for the breeding of Angus cattle and the construction of core Angus cattle population in Ningxia.