Abstract: The study aimed to obtain the relevant information on the biological activity of papaya enzyme, from the chemical composition content, efficacy enzyme activity and antioxidant activity of papaya enzyme. The results showed that the contents of total sugar, total phenol, total flavonoids and protein of papaya enzyme were（3 090.00±193.25）,（161.67±4.62）,（55.00±1.00）,（134.67±1.15） mg/L, respectively. The enzyme activities of amylase, lipase,protease and total superoxide dismutase（T-SOD） of papaya enzyme were（0.09±0.026）,（7.11±1.08）,（0.64±0.127）,（67.68±2.244） U/m L, respectively. The IC50 value of papaya enzyme to 1,1-diphenyl-2-picrylhydrazyl-1-（2,4,6-trinitrophenyl） hydrazyl（DPPH） radical, hydroxyl radical and superoxide anion radical were 6.70%, 28.91% and42.30%, respectively. The total antioxidant activity of papaya enzyme was（30.12±0.167） U/m L. The development of this research will improve the recognition of papaya enzyme and promote the development of the fruit and vegetable enzyme industry.