Abstract: B. oleracea, B. napus and B. rapa are important economic crops, belonging Brassica, and the relationship between them can be expressed in the theory of Yu’s triangle. CAMTA is a family of transcription factors that are highly conserved during evolution. This family member plays an important role in the stress resistance of Brassica plants. In order to investigate the function of the CAMTA3 gene family of Brassica, this study used bioinformatics to identify eight genes of the CAMTA3 gene family of Brassica（B. napus, B. rapa, B. oleracea） and evolved from the physicochemical properties and family of proteins. Analytical studies were carried out with regard to gene structure and upstream promoters. The analysis showed that the members of the eight CAMTA3 gene families were highly conserved during the evolution process, and their genetic relationship was consistent with the theory of Yu’s triangle. At the same, each member played an important role in plant response to stress. The results provided a certain information basis for the functional study of the CAMTA3 gene family in Brassica.