Abstract: In order to clone the MiSuSy（MiSS） gene sequence of mango（Mangifera indica L.）, forecast MiSuSy biological characteristics and illuminate its expression characteristics and the function in the development of the fruit,in this study, homology cloning and rapid amplification of cDNA ends（RACE） technology were used to cloned the full-length cDNA of MiSuSy（MiSS） with the length of 2 110 bp, which contained an opening reading frame of 1 455 bp.This gene encoded a protein of 484 amino acids with a theoretical molecular weight of 55.3 kD and an isoelectric point of 6.08. Phylogenetic analysis showed that the amino acid sequences of MiSS were 90%～93% indentical to the sequence of Citrus unshiu, Litchi chinensis and Dimocarpus longan. RT-qPCR analysis showed that the expression level of MiSS gene increased first and then decreased, whereas the amount of MiSS gene expression in the exocarp was significantly higher than that in mesocarp of all stages in fruit development. It was reasonable to infer that MiSS gene may be closely related to starch synthesis. This study provided a foundation for understanding the role S of MiSS gene in sucrose metabolism and the mechanism of plant growth regulator affecting sugar metabolism from molecular perspective.