Abstract: This study aimed to obtain the full length of geranyl diphosphate synthase（GPS） gene（AlGPS） from Atractylodes lancea, and clarify its structural characteristics. The specific primers were generated based on the previous trancriptome sequences, and used for PCR amplification to obtain the full length cDNA sequence of AlGPS.The open reading frame of AlGPS was 1 266 bp encoding 421 amino acids（GenBank accession number: MH5227-14.1）. The results of bioinformatics analysis showed that the AlGPS had the highest similarity to the GPS from Artemisia annua, both of which contained the domain of isoprenoid synthase superfamily and geranyl diphosphate synthase superfamily. AlGPS protein was non-secreted protein, and its transmembrane structure was predicted to be intramembrane protein. Subcellular localization indicated that the protein was probably present in the chloroplast. The AlGPS gene sequences and biological characteristics obtained in this study provide references for terpene biosynthesis and regulation in Atractylodes lancea, and will further facilitate the study on the biological function of AlGPS and molecular breeding in A. lancea.