Abstract: miR-199 a-5 p is a member of miRNAs family. To explore the effect on activation, proliferation and migration of human hepatic stellate cells and provide a new idea for clinical treatment of hepatic fibrosis, in this study, the overexpression vector of miR-199 a-5 p was constructed and the antisense oligonucleotide（ASO） of miR-199 a-5 p was synthesized and transfected into LX-2 cells by liposome. The Cell Counting Kit-8 kit and Transwell were used to detect cell proliferation and migration ability; the colony formation experiment was used to detect the colony forming ability of LX-2 cells; real-time quantitative PCR was used to detect the mRNA expression levels of the fibrosis-related genes α-SMA and CollagenⅠafter miR-199 a-5 p transfection in the cells; Western blot was used to detect the α-SMA and Collagen I protein levels in each group. The results showed that miR-199 a-5 p can promote the proliferation（P＜0.01）, migration ability（P＜0.01） and colony formation ability（P＜0.01） of LX-2 cells,while inhibiting the expression of miR-199 a-5 p can inhibit cell proliferation, cell migra tion abilities and colony formation ability（P＜0.01）. The RT-qPCR results showed that the expression level of miR-199 a-5 p in LX-2 cells that were treated with TGF-β1 were higher than that without TGF-β1 treated（P＜0.05）, over-expression of miR-199 a-5 p could make both the α-SMA mRNA（P＜0.01） and protein（P＜0.05） expression levels increased. These conclusions indicated that miR-199 a-5 p could promote the cells activity and proliferation ability of LX-2 cells.