Abstract: GINS2 is up-regulated in a variety of invasive tumors. However, it’s role and function in non-small cell lung cancer（NSCLC） remains to be discoverd. This study focused on the role and functional analysis of GINS2 in non-small cell lung cancer. Through the analysis of tumor genome atlas（TCGA） database, it was found that GINS2 was significantly up-regulated in NSCLC. In order to study the role and function of GINS2 in NSCLC cells,firstly, siRNA of GINS2 was designed by using small interfering RNA technology to silence GINS2 mRNA. After transfection at the cell level, the expression of GINS2 gene decreased in NCI-H292 and A549 cell lines of non-small cell lung cancer. Subsequently, Real Time Cell Analyzer（RTCA） and Transwell Cell assay were used to measure cell proliferation, migration and invasion. Finally, Cell cycle and apoptosis were analyzed by flow cytometry. The results confirmed that after the transfection of siRNA in two cell lines, NCI-H292 and A549, the proliferation, migration and invasion ability of GINS2 were significantly inhibited. In addition, cell cycle apoptosis experiments showed that in NCI-H292 cell line, the transition from G1 phase to S phase was blocked. In the A549 cell line, the transformation from S phase to G2 phase was blocked, and the cell apoptosis was promoted in both cell lines. In conclusion, knocking down GINS2 can inhibit the proliferation, migration and invasion of NCI-H292 and A549 cells, block the cycle and promote apoptosis, etc. Therefore, the study of the function of this gene in NSCLC is of great significance for the follow-up study on the molecular mechanism, which may be a specific therapeutic target in the future.