Abstract: Zinc finger GATA like protein-1（ZGLP1） is a nuclear zinc finger protein that regulates the interaction between germ cells and somatic cells during gonad developmental process in mammals. In order to study the function of ZGLP1 in fish, we took Oryzias curvinotus as the research object and carried out PCR amplification,molecular cloning and sequence characterization analysis of the zglp1 gene, and the spatiotemporal expression pattern was detected by reverse transcription-PCR. The results showed that two zglp1 transcripts（orcuzglp1 x1 and orcuzglp1 x2） were amplified from cDNA of O. curvinotus ovary tissue. Orcuzglp1 x1 was 1 739 bp in lengthand contained an open reading frame of 1 665 bp. It was predicted that the encoded protein contained 554 amino acids and the molecular weight of the protein was 61.40 kDa. Orcuzglp1 x2 was 1 629 bp in length, and the length of the open reading frame was 1 407 bp. The encoding protein contained 468 aa, and the molecular weight of the protein was51.81 kDa. Orcuzglp1 x2 had a deletion of 86 aa at the N terminal compared with Orcuzglp1 x1, and orcuzglp1 s had two zinc finger structures（Znf1, Znf2） at the C terminal. Znf1 was highly homology with the typical GATA zinc finger DNA binding motif. The results of multi-sequence alignment showed that Znf1 was more conserved among species,while Znf2 was less conserved. Phylogenetic tree analysis showed that Zglp1 was s consistent with species evolution.RT-PCR results showed that orcuzglp1 s was mainly expressed in mature ovarian tissues and early embryonic development（cleavage stage）. In conclusion, the O. curvinotus zglp1 may play a role in oogenesis and embryo cleavage.