Abstract: The purpose of this experiment was to analyze the molecular characteristics and expression patterns of the myocyte enhancer factor 2 C（MEF2 C） gene of yak（Bos grunniens）, and explore the mechanism of MEF2 C gene influencing yak muscle development. In the experiment, muscle tissue cDNA of Datong yak was used as a template and MEF2 C gene sequence was amplified throuth PCR amplification technology and its encoded protein structure were analyzed by using DNAStar, ExPASy, ABCpred, and other bioinformatics software. The expression of MEF2 C gene was detected by real-time fluorescence quantitative PCR（RT-qPCR） technology. The yak MEF2 C gene coding region obtained by cloning was 1 302 bp in length, encoding 433 amino acids. The protein structure prediction results showed that the MEF2 C protein with a half-life of 30 h, was a hydrophilic basic protein, having no signal peptide but has a transmembrane structure. In the system relationship, the yak was closest to the ordinary cattle, but farther away from the mouse. The results showed that MEF2 C gene was expressed in 7 tissues of yak, and the highest expression was found in muscle tissues, the MEF2 C gene expression in different periods was fetal yak＞ adult yak＞ 6 months old yak.The research results will provide a scientific basis for further exploring the role of MEF2 C gene in yak muscle development, and also provide data support for the analysis of the molecular mechanism of yak muscle development.