粗纤维对定安鹅MSTN基因表达量及肌纤维发育的影响
Effects of Crude Fiber on Muscle Fiber Development and MSTN Gene Expression in Ding’an Goose
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摘要: 为研究日粮粗纤维水平对生长中期定安鹅腿肌MSTN基因表达量及肌纤维发育的影响,选遗传背景相近、体重一致的30日龄定安鹅120只,采用单因子随机试验设计,共分3个处理组,每处理组设4个重复,每重复10只鹅。分别饲喂粗纤维水平为4.29%,5.29%和6.29%的三种日粮。试验期为35 d。结果表明:(1)日粮粗纤维水平为5.29%时,可以提高生长中期定安鹅试验末重和平均日增重,并且与6.29%组比较达到了显著水平(P<0.05);(2)日粮粗纤维水平可以显著影响生长中期定安鹅腿肌中MSTN的基因表达量(P<0.05),且腿肌中MSTN的基因表达量与生长中期定安鹅的试验末体重和平均日增重呈负相关关系;(3)日粮粗纤维水平为5.29%时,与6.29%组相比可显著提高生长中期定安鹅血清中Ig A和Ig G的含量(P<0.05);(4)日粮粗纤维水平显著影响生长中期定安鹅腿肌纤维数量和腿肌纤维密度的影响(P<0.05)。综上所述,日粮粗纤维水平为5.29%可以显著影响生长中期定安鹅的试验末体重、平均日增重和腿肌中MSTN基因的表达水平,对腿肌纤维的发育也有一定的促进作用。Abstract: The experiment was conducted to study the effects of dietary crude fiber level on muscle fibers development and MSTN genes expression of Ding’an goose at the middle growth stage. 120 30-day-old Ding’an geese with similar genetic background and the same weight were selected and divided into three treatment groups with four replicates in each treatment group. Each repeat was 10. Three diets with crude fiber levels of 4.29%, 5.29%and 6.29% were fed respectively. The experimental period was 35 days. The results showed that:(1) The end weight and average daily gain of 5.29% group significantly increased compared with those of 6.29% group(P<0.05);(2) The leg muscle MSTN gene express level of mid-growth stage Ding’an goose was significantly effected by dietary crude fiber(P<0.05), and the leg muscle MSTN gene expression level was negatively correlated with the body weight and average daily gain of mid-growth stage Ding’an goose;(3) Serum Ig A and Ig G levels of 5.29%group significantly increased compared with those of 6.29% group(P<0.05);(4) The number of leg muscle fibers and the density of leg muscle fibers of middle growth stage Ding’an goose were significantly affected by the dietary fiber levels(P<0.05). In conclusion, dietary crude fiber level of 5.29% group could significantly affect the end weight, average daily gain and the leg muscle MSTN expression level of the middle growth stage Ding’an goose,and promote the leg muscle fiber development.