Abstract:
Endogenous NO, CO and H2 S are the three gas signal molecules currently discovered, which play an important role in vasoconstriction regulation and vascular remodeling reactions, affecting hypoxic pulmonary vasoconstriction(HPV). In this experiment, the altitude of 5 000 m was simulated, and the plateau pika(Ochotona curzoniae) and the SD rat were treated with hypoxia at different times, to measure the mean pulmonary artery pressure, right heart index, and the concentration of endogenous NO, CO, and H2 S in the blood to reveal the role of three gas molecules in hypoxic adaptation of plateau pika. The results showed that:(1) With the increase of hypoxic time, there was no significant difference in pulmonary artery pressure of plateau pika(P>0.05), and there was no significant change in right heart index(P>0.05). Pulmonary arterial pressure in SD rat was hypoxia. There was a very significant increase in 7 days(P<0.01), and the pulmonary artery pressure increased with the increase of hypoxic time, and the right heart index showed a significant increase(P<0.01);(2) The levels of CO in the blood of plateau pika and SD rat remained relatively stable between treatments(P>0.05). The NO concentration in the blood of plateau pika significantly increased at 7 days of hypoxia(P<0.01), and the NO concentration returned to normal level at21 days of hypoxia(P>0.05). SD rat was in the blood with hypoxia for one day. The concentration of NO decreased significantly(P<0.05), and the NO concentration returned to normal level at 7 and 21 days of hypoxia(P>0.05).There was no significant difference in H2 S concentration in the blood of plateau pika at one day of hypoxia(P>0.05), and there was a significant increase in H2 S concentration at 7 and 21 days of hypoxia(P<0.01). SD rat was exposed to hypoxia for one day. The concentration of H2 S in the blood increased significantly(P<0.01), and the concentration of H2 S in hypoxic 7 days and 21 days decreased significantly compared with the normal level(P<0.05).The results indicated that increasing the level of H2 S in the blood of plateau pika may play an important role in maintaining normal pulmonary artery pressure and oxygen transmission.