Abstract: Transcription factors PU.1 and KLF7 play important roles in adipogenesis, hematopoiesis, tumorigenesis, and body immune, but whether they are functionally related is unclear. The purpose of this study was to investigate the expression pattern and correlation of PU.1 and KLF7 in different tissue of mices. The expressions of PU.1 and KLF7 in the stomach, liver, jejunum, lung, leg muscle, heart, fat, chest muscle, spleen, kidney and brain tissues of mice were detected by RT-qPCR, and the correlation was analyzed. The results showed that KLF7 was highly expressed in lung, stomach and fat, and PU.1 was highly expressed in lung, liver and spleen. The expression of the two genes was significantly positively correlated（r=0.857, P=0.001）. Bioinformatics prediction showed that there were 5 binding sites of PU.1 located in the 5’-flanking region of KLF7, which may regulate the expression of KLF7 gene. This study provided important basic data for in-depth study of the function and interaction mechanism of PU.1 and KLF7.