Abstract: Parrots are charismatic birds, their plumage and capacity for learning make them highly sought-after pets. In recent years, the illegal trade in parrots has posed a serious threat to the viability of native populations. To reduce the logistical difficulties associated with trafficking live birds, smugglers often transport eggs. This creates a problem in elucidating accurate species identification. Based on the 97 parrot eggs seized by the Beijing Customs Anti-smuggling Bureau, this study explored the parrot species identification rate based on the four barcodes of mitochondrial genes Cyt b, COⅠ, 12 S rRNA and 16 S rRNA. According to the existing reference database, 81% of the parrot eggs can be identified to the species level, and the remaining 19% of the eggs can only be identified to the level of the family or genus. In this study, the existing mitochondrial gene barcodes and their mitochondrial genome of Psittaciformes were fully statistically analyzed. The result showed that there are 77.33% species of Psittaciformes had mitochondrial genes Cyt b, COⅠ, 12 S rRNA, 16 S rRNA sequence data in the current database of GenBank. The lack of mtDNA sequences in the reference database posed a major obstacle to the accurate identification of species. This study aimed to provide a reference for relevant law enforcement agencies and identification agencies by counting and evaluating the mtDNA barcodes of the parrots in the reference database.