Abstract: Magnaporthe oryzae is the most harmful pathogen in the world for rice and the main model organism for elucidating the molecular basis of plant fungal disease. Carrying out relevant research of the growth and development of Magnaporthe oryzae has a great significance for the control of Magnaporthe oryzae. MGG＿14095 gene is the pathogenic gene of Magnaporthe oryzae. The bioinformatics prediction of MGG＿14095 protein showed that the isoelectric point（pI） of MGG＿14095 protein was 5.72, and the instability index was 50.99, belonging to the acid unstable protein and the α/β hydrolase superfamily, containing the keratinase conserved domain; having obvious transmembrane structure and signal peptide cutting site, belonging to the secretory protein. In this study, we cloned the MGG-14095（38-281） gene of Magnaporthe oryzae, constructed the prokaryotic expression system pETM20-MGG＿14095（38-281）, used the stepwise chromatography to purify MGG＿14095（38-281） protein, and the high-purity soluble target protein was obtained successfully. The results provided certain theoretical and experimental basis for the analysis of MGG＿14095（ 38-281） protein structure, the exploration and the control of pathogenetic mechanism of Magnaporthe oryzae.