Abstract: The aim of this study was to select Akebia trifoliata（Thunb.） Koidz. var. australis（Diels） Rehd.germplasm with lower and more stable ratio of glucose content/D-mannose content（CG/CD） by evaluating Akebia trifoliata（Thunb.） Koidz. var. australis（Diels） Rehd. germplasm resources based on the content of D-mannose and glucose. D-mannose and glucose content of peels in different A. trifoliata（Thunb.） Koidz. var. australis（Diels）Rehd. germplasms was measured by RP-HPLC system. The results showed that D-mannose content of A. trifoliata（Thunb.） Koidz. var. australis（Diels） Rehd. germplasm-’Qiantong No.1’ was the highest in the same regional test site and different regional test sites and with lower CG/CDratio in 2 years. However, the result difference was major and showed significant and highly significant difference in different regional test sites; D-mannose and glucose content of A. trifoliata（Thunb.） Koidz. var. australis（Diels） Rehd. germplasm-’Qiantong No.2’ and ’Qiantong No.3’ showed unstable state in the same regional test site and different regional test sites. The effect of environmental factors to the content of D-mannose and glucose of ’Qiantong No.1’,’Qiantong No.2’ and ’Qiantong No.3’ was greater than that of the genetic factors itself. The stability of D-mannose and glucose metabolism of ’Qiantong No.1’ was stabler than that of ’Qiantong No.2’, ’Qiantong No.3’, which was more suitable for planting as medicinal germplasm.