Abstract: Breast cancer is the most common malignant tumor in women worldwide. Accurate early diagnosis and prognosis biomarkers can improve the efficiency of treatment. Apoptosis-associated specklike protein（ASC） is a cohesive protein that plays an important role in oncogenesis. In order to further explore the mechanism of ASC, the expression level of PYCARD was assessed by using Oncomine analysis. MethHC database was used to examine the methylation status of PYCARD. The expression of PYCARD and its prognostic value in breast cancer were evaluated by using bc-GenExMiner v4.3, PYCRAD gene was associated with clinicopathological parameters in breast cancer patients, and the PrognoScan database was used to analyze the relationship between PYCARD, prognosis of breast cancer patients. PYCARD gene was highly expressed in 8 cases of breast cancer, and PYCARD methylation level in cancer tissues was significantly higher than that in normal tissues. Prognostic analysis showed that patients with high PYCARD m RNA expression had higher overall survival（OS）, distant metastasis free survival（DMFS）, and relapse free survival（RFS） than those with low m RNA expression, and the prognosis results showed that the survival rate of patients with high expression was higher than that of low expression, which was likely to be related to the methylation level of PYCARD gene. It had certain clinical significance in guiding breast cancer treatment and evaluating prognosis.