Abstract: Selenium, as a necessary trace element for humans and animals, can regulate the levels of lipid and glucose metabolism related genes in pigs, lambs, mice, humans and yeast at different concentrations. However, the effect of low dose of selenium on lipid and glucose metabolism in HepG2 cell is unclear, The real time PCR and genomics analysis were performed to evaluate the changes of lipid and glucose metabolism related genes in sodium selenite treated HepG2 cells. The results suggested that the lipid metabolism related genes, FAR1, DGKD and HILPDA, increased at 24 h or 36 h, and the glucose metabolism related genes, UGDH, IRS-2 and PEPCK, also increased at 24 h and 36 h, compared with those at 0 h. And 0.1 μmol/L sodium selenite could only increase the expression levels of TRAP1, HILPDA and PEPCK at 24 h, which of them play an important role in tumor development. Moreover, transcriptomics analysis suggested that selenium treatment could change the metabolic regulatory network, including terpenoid backbone biosynthesis, indicated resistance, inositol phosphate metabolism and cell cycle pathways. The above results indicate that 0.1 μmol/L sodium selenite could influence lipid and glucose metabolism in HepG2 cells.