Abstract: In order to investigate the effect of curcumin on human exercise-induced oxidative stress. In this study,30 male subjects were selected for the study, and all subjects completed 3 trials in random order:（1） placebo group（Control）,（2） single curcumin group（only before exercise, Single） and（3） double curcumin group（before and after exercise, Double）. Subjects received 100 mg of curcumin or placebo 2 hours before exercise and immediately after exercise. Subjects ran for 60 min on a treadmill with 65% VO2 Max. Blood samples were collected before exercise, after exercise, and after 2 hours of exercise to detect oxidative stress and antioxidant activity indicators.Studies have shown that serum d-ROMs concentrations and plasma TRX-1 concentrations after exercise were significantly lower than that of before exercise in subjects who received curcumin（P＜0.05）. After exercise, serum BAP concentrations in subjects who received curcumin were significantly higher than that of before exercise（P＜0.05）. After 2 hours of exercise, the plasma GSH concentration of subjects who received curcumin for a single time was significantly higher than that of before exercise（P＜0.05）. The results showed that the intake of curcumin can attenuate exercise-induced oxidative stress by increasing the body’s antioxidant capacity.