缢蛏HSP90基因的鉴定及其在不同盐度下的表达分析

叶博; 李燕; 牛东红; 李家乐

doi:10.13417/j.gab.040.002414

缢蛏HSP90基因的鉴定及其在不同盐度下的表达分析

叶博¹,
李燕¹,
牛东红^1,2,3, ,,
李家乐^1,2,3

1. 上海海洋大学水产种质资源发掘与利用教育部重点实验室;
2. 上海海洋大学水产动物遗传育种中心上海市协同创新中心;
3. 上海海洋大学水产科学国家级实验教学示范中心

基金项目:

国家重点研发计划“蓝色粮仓科技创新”专项(2019YFD090040)

国家自然科学基金项目(31472278)共同资助

详细信息

通讯作者:
牛东红,dhniu@shou.edu.cn

中图分类号: S917.4
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出版历程
- 刊出日期: 2021-08-24

Identification of HSP90 gene in Sinonovacula constricta and Its Expression Analysis at Different Salinity Concentration

YE Bo¹,
LI Yan¹,
NIU Dong-hong^1,2,3, ,,
LI Jia-le^1,2,3

1. Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources,Shanghai Ocean University;
2. Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding,Shanghai Ocean University;
3. National Demonstration Center for Experimental Fisheries Science Education,Shanghai Ocean University

摘要

摘要: HSP90是一种高度保守的特异性蛋白，在增强动物对逆环境的适应能力方面发挥重要作用。本研究从缢蛏(Sinonovacula constricta)转录组文库中筛选出热休克蛋白90 (heat shock protein 90, HSP90)的一条同源序列，通过RACE技术获得其c DNA全长序列，命名为ScHSP90。序列分析表明，该基因cDNA全长3 070 bp，包括428 bp的5'非翻译区，461 bp的3'非翻译区和2 181 bp开放阅读框，编码一个由726个氨基酸组成的多肽。该多肽含有HSP90家族共有的5个签名序列和C端高度保守的MEEVD短肽序列。同源性及系统分析表明，ScHSP90编码的氨基酸与其他物种的HSP90氨基酸序列具有很高的同源性，其中与软体动物HSP90氨基酸序列相似性达80%以上。荧光定量RT-qPCR检测分析表明：ScHSP90基因在成体各组织中均有表达，在水管和肝胰腺中表达量相对较高；在不同盐度条件下的急性实验中，经盐度30 ppt和35 ppt处理后ScHSP90基因表达水平极显著升高(P<0.01)，且盐度越高上调越明显，表明ScHSP90基因参与了机体适应逆环境的过程。本研究为进一步研究HSP90基因功能以及开展贝类抗逆选育提供一定的理论参考。
- 缢蛏 /
- HSP90 /
- 盐度 /
- 基因表达
Abstract: Heat shock protein 90（HSP90） is a highly conserved specific protein that plays an important role in enhancing the animal’s ability to adapt to adverse environments. In the present study, we obtained HSP90 sequence from the transcriptome of Sinonovacula constricta and cloned the full length cDNA, named as ScHSP90. The full length of ScHSP90 cDNA was 3 070 bp, including a 428 bp 5’ UTR, a 461 bp 3’ UTR and a 2 181 bp ORF encoding a polypeptide of 726 amino acids. It contained five signature sequences of HSP90 family and a highly conserved short peptide MEEVD in the C-terminus. The sequence analysis showed that the amino acid sequence similarity with the mollusc HSP90 was more than 80%. Real-time quantitative PCR（RT-qPCR） analysis showed that ScHSP90 could be expressed in all examined tissues and the relative higher expression level was observed in siphon and hemolymph. After the acute treatments of different salinity concentration, the expression of ScHSP90 gene significantly increas-ed at salinity 30 ppt or 35 ppt（P＜0.01）, and the expression increased following the salinity concentration increasing. It is speculated that the ScHSP90 gene could be involved in the process of adapting the adverse environment. It provides a theoretical reference for further study of HSP90 gene function and breeding of shellfish.
- Sinonovacula constricta /
- HSP90 /
- Salinity /
- Gene expression

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