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中华绒螯蟹(Eriocheir sinensis)全基因组微卫星分布特征研究

Study on Distribution Characteristics of Whole Genome Microsatellite of Eriocheir sinensis

  • 摘要: 本研究利用MISA软件对中华绒螯蟹(Eriocheir sinensis)全基因组微卫星进行了筛选并对其分布特征进行了深入分析。结果显示:在中华绒螯蟹全基因组中,共筛选出886 782个完整型微卫星,相对丰度为572个/Mb。总长度为14 620 825 bp,占全基因组序列长度的0.94%,相对密度为9 438 bp/Mb。在六种完整型微卫星中,单碱基微卫星的数目最多,共有595 093个,占基因组中微卫星总数的67.11%;其他依次为二碱基(19.28%)、三碱基(11.73%)、四碱基(1.56%)、五碱基(0.30%)和六碱基(0.03%)类型微卫星。中华绒螯蟹全基因组中前十种优势微卫星分别为A,AC,AG,AGG,C,AT,ACT,ACC,AAT和AAG。本研究为深入研究中华绒螯蟹基因组特征以及进一步开发微卫星引物等提供了数据支持。

     

    Abstract: In this study, microsatellite-searching software MISA was used to screen and analyze microsatellites in the genome of Chinese mitten crab(Eriocheir sinensis). The results were showed that in the whole genome of E.sinensis, 886 782 microsatellite sequences were discovered with a relative abundance of 572/Mb. The total length was 14 620 825 bp, accounting for 0.94% of the genome sequence, and the relative density was 9 438 bp/Mb. Among the 1~6 microsatellite repetitions, mononucleotide had the largest numbers with a total of 595 093, accounting for 67.11% of all microsatellites. The others were dinucleotide(19.28%), trinucleotide(11.73%), tetranucleotide(1.56%), pentanucleotide(0.30%) and hexanucleotide(0.03%). The ten dominant microsatellite classes in the whole genome of E. sinensis were A, AC, AG, AGG, C, AT, ACT, ACC, AAT and AAG, respectively. This study provided data support for further research on the genomic characteristics of E. sinensis and further development of microsatellite primers.

     

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