Abstract: In this study, microsatellite-searching software MISA was used to screen and analyze microsatellites in the genome of Chinese mitten crab（Eriocheir sinensis）. The results were showed that in the whole genome of E.sinensis, 886 782 microsatellite sequences were discovered with a relative abundance of 572/Mb. The total length was 14 620 825 bp, accounting for 0.94% of the genome sequence, and the relative density was 9 438 bp/Mb. Among the 1～6 microsatellite repetitions, mononucleotide had the largest numbers with a total of 595 093, accounting for 67.11% of all microsatellites. The others were dinucleotide（19.28%）, trinucleotide（11.73%）, tetranucleotide（1.56%）, pentanucleotide（0.30%） and hexanucleotide（0.03%）. The ten dominant microsatellite classes in the whole genome of E. sinensis were A, AC, AG, AGG, C, AT, ACT, ACC, AAT and AAG, respectively. This study provided data support for further research on the genomic characteristics of E. sinensis and further development of microsatellite primers.