Abstract:
The differential genes affecting meat quality and taste were screened by RNA-seq, and the expression of genes related to meat taste in breast muscle and leg muscle of Jingyuan chicken were verified by RT-qPCR. The results showed that there were 666 genes significantly differentially expressed between breast muscle and leg muscle of Jingyuan chicken, of which 256 genes were significantly up-regulated and 410 genes were significantly down-regulated(P<0.05). GO functional annotation and KEGG pathway analysis showed that adenylosuccinate synthetase isozyme 1(ADSSL1), a differentially expressed gene related to inosinic acid, was screened from purine metabolism, alanine, aspartic acid and glutamate metabolism pathways, and the m RNA expression of ADSSL1 in chest muscle was significantly higher than that in leg muscle(P<0.01). The results of this study provide a scientific basis for the screening of inosinic acid-related candidate genes, and provide a theoretical reference for further understanding of ADSSL1 genes.