Abstract: The aim of this study was to obtain the Cr ogOBP1 gene of Crematogaster rogenhoferi by cloning,through the method of bioinformatics and molecular biology to implement analytical prediction of the signal peptide and secondary structure. The expression specificity of CrogOBP1 in head, antennae, chest, abdomen and foot were determined by fluorescence quantitative RT-PCR. The results showed that the full-length of open reading frame of CrogOBP1 was 420 bp, encoding a signal peptide of 139 amino acids with 20 amino acid residues. It had six conserved cysteine sites and other characteristics of odorant binding proteins. It was predicted that the molecular weight of the encoded protein was 15.4 KD and the isoelectric point was 4.13. CrogOBP1 was closely related to OBP69 a of Pogonomyrmex barbatus and OBP69 a of Vollenhovia emeryi. It was highly expressed in the antennae which was significantly higher than other tissues. This study provide a theoretical basis for further understanding of the function and the control of Parasaissetia nigra Nietner by means of chemical ecology.