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长角血蜱蛋白质二硫键异构酶-2的生物信息学分析

Bioinformatics Analysis of PDI-2 Immunogenic Protein from Haemaphysalis longicornis

  • 摘要: 本研究旨在利用生物信息学软件分析长角血蜱的蛋白质二硫键异构酶(protein disulfide isomerase-2,PDI-2)的结构特点,并预测其B、T细胞抗原表位。利用ProtParam分析PDI-2蛋白质的理化性质,利用SignalP分析蛋白的信号肽,利用SOSUI分析蛋白可溶性,利用Bcepred分析蛋白表面可及性、柔韧性和亲(疏)水性,利用ProSacle分析蛋白亲水性区域,利用MotifScan分析蛋白翻译后修饰位点,利用SOPMA分析蛋白的二级结构,利用Phyre2预测蛋白质三级结构,利用IEDB、BepiPred、SYFPEITHI预测其T、B细胞抗原表位。结果显示,长角血蜱PDI-2的分子式为C2538H3966N654O795S17,理论等电点为4.85;PDI-2含有13个(scores≥1.9)高亲水性区域,11个(scores≥2.0)柔韧性区域,11个(scores≥1.9)表面可及性区域;该蛋白含有20个蛋白翻译后修饰位点;α-螺旋、β-折叠、β-转角和无规则卷曲分别占42.69%、19.44%、5.01%和32.87%;最终预测PDI-2蛋白存在20个CTL优势表位和3个Th优势表位,可能的B细胞抗原表位有23个。本研究发现PDI-2蛋白含有丰富的抗原表位,为深入研究该蛋白提供了理论依据。

     

    Abstract: This study was aimed to analyze the PDI-2 protein of Haemaphysalis longicornis by using bioinformatics software and to predict possible epitopes of its B cells and T cells. The physicochemical properties of the protein were analyzed by ProtParam; the secondary structure and tertiary structure of the protein were predicted by SOPMA and SWISS-MODE; the B. cell epitope was predicted by IEDB and BepiPred; and the T cell epitope was predicted by SYFPEITHI. After comparison, the protein formula of long-horned blood clam PDI-2 is C2538 H3966 N654 O795 S17,the theoretical isoelectric point was 4.85, and there were three transmembrane regions. PDI-2 contained 13 regions with a high hydrophilicity parameter score of ≥1.9, 11 regions with a flexibility parameter score of ≥2.0, and 11 regions with a surface accessibility parameter score of ≥1.9. The protein contained 20 post-translational modification sites, with α-helix of 42.69%, β-sheet of 19.44%, β-turn of 5.01%, and random curl of 32.87%. There were 20 CTL dominant epitopes, 3 dominant epitopes and 23 B cell dom inant epitopes. After analysis and comparison, PDI-2 protein was rich in antigenic epitopes, providing a scientific theoretical basis for the study of aphid vaccines.

     

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