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澄黄滨珊瑚微卫星标记的开发

Development of Microsatellite Markers for the Hermatypic Coral Porites lutea

  • 摘要: 本研究通过挖掘GenBank中滨珊瑚属(Porites)近缘种的EST和基因组微卫星序列资源,使用种间扩增和重测序技术,开发了澄黄滨珊瑚(Porites lutea)-虫黄藻(Symbiotic zooxanthellae)共生体的微卫星标记,并通过纯的共生虫黄藻DNA的扩增验证,确认了20个澄黄滨珊瑚微卫星标记和43个共生虫黄藻微卫星标记。在海南岛岸礁八所群体(BS, n=16)中测试扩增,有19个标记呈现多态性。多态位点的等位基因数为2~7 (平均3.5±1.8),观测杂合度(H_o)和期望杂合度(He)分别为0.06~0.94 (平均0.34±0.29)和0.18~0.83 (平均0.57±0.20)。由于存在无效等位基因,4个位点在经Bonferroin多重比较校正后仍然偏离Hardy-Weinberg平衡(PHWE<0.05)。这是澄黄滨珊瑚的首套微卫星标记,它们可用于对该物种遗传多样性和连通性的研究,从而促进印度洋-太平洋海区濒危的珊瑚礁生态系统及造礁珊瑚群落的保护。

     

    Abstract: By mining the EST and genomic microsatellite sequence resources of Porites species in GenBank, and using interspecific amplification and resequencing, we developed the microsatellite markers for Porites lutea symbiont, and by identifying with pure zooxanthella DNA, 20 P. lutea microsatellite markers and 43 microsatellite markers of its symbiotic zooxanthella were validated. These markers of P. lutea were genotyped in one wild population(n=16) in the southwest of Hainan Island fringing reef. Nineteen of the 20 markers showed polymorphism.The number of alleles was 2~7(mean 3.5±1.8), and the observed heterozygosity(Ho) and expected heterozygosity(He) were 0.06~0.94(mean 0.34±0.29) and 0.18~0.83(mean 0.57±0.20), respectively. Four markers remained deviated from the Hardy-Weinberg Equilibrium(HWE) after multiple comparison correction for Bonferroin(PHWE<0.05), probably due to the presence of null alleles. This is the first set of microsatellite markers for P. lutea. They should be useful for the research on the genetic diversity and connectivity of this species and the conservation of endangered coral reef ecosystems and reef-building coral communities in the Indo-Pacific region.

     

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