Abstract:
The genes of odorant binding protein(OBPs) and chemosensory protein(CSPs) were identified by transcriptome sequencing of the antennae of Carposina sasakii, which provided the genetic resources for the further study on the functions of these two kinds of olfactory proteins. The cDNA libraries of C. sasakii antennae were sequencing using an Illumina Hiseq 4000 platform. The OBPs and CSPs genes were identified by comparing them with the characteristic sequences of OBPs and CSPs from the other insects. The evolutionary relationships between the OBPs and the CSPs of C. sasakii and those of the other Lepidopteran insects by constructing neighbor joining trees. The study obtained 67 060 329 clean reads. 82 426 transcripts were generated after the de novo assemblies of the clean reads data, and finally generated 63 327 unigenes after removed redundant transcripts. Using keyword searching and PSI-BLAST comparing, 26 OBPs and 16 CSPs genes of C. sasakii antennae were identified. The results of sequence alignment showed that the sequence similarities among different OBPs or different CSPs of C. sasakii antennae were very low, ranging from 10.00% to 78.57% and 20.47% to 75.20%, respectively. The results of phylogenetic analyses showed highly differentiations among different OBPs or among different CSPs. Most of the genes were clustered into different evolutionary branches. In this study, we obtained a large number of OBPs and CSPs genes in the antennae of of C. sasakii, providing a basic guarantee for further study on the molecular mechanisms of peripheral olfactory proteins of this insect.