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bta-miR-103在牛各组织中的表达及其靶基因预测

The Expression of bta-miR-103 in Bovine(Bos taurus) Tissues and Predicted Target Genes

  • 摘要: miRNA作为内源性小的单链非编码RNA,在组织器官的发育和发生、细胞增殖和凋亡等方面起着重要作用。鉴于目前尚不完全明确bta-mi R-103在牛各组织中的表达规律及其可能的靶基因,本研究首先对bta-miR-103在牛基因组中的位置及序列信息进行了分析,并通过茎环荧光定量PCR法检测了牛不同组织中bta-miR-103的表达情况,结果表明其在牛上皮组织中的表达量最高,其次是骨髓和结缔组织。为了进一步了解bta-miR-103的调节作用及其机制,使用TargetScan等在线软件对其可能结合的靶基因进行了预测,发现存在744个基因mRNA的3'UTR可能和bta-mi R-103结合,并且大部分靶基因参与细胞的增殖、分化和凋亡。本研究结果可为深入研究bta-miR-103在牛各组织中增殖和分化的作用奠定基础。

     

    Abstract: microRNAs(miRNAs) as small endogenous single-stranded non-coding RNAs play important roles in the development and occurrence of tissues and organs, cell proliferation and apoptosis. However, the expression patterns of bta-miR-103 in cattle tissues and its possible target genes are not yet fully understood. In this study, the position and sequence information of bta-miR-103 in the cattle genome were firstly analyzed, then the expression of bta-miR-103 in different bovine tissues were also detected by Stem-loop real-time PCR. The results showed that the expression of bta-miR-103 was highest in bovine epithelial tissues, followed by bone marrow and conne ctive tissue. In order to understand the role and mechanism of bta-mi R-103, the candidate target genes were predict ed using TargetScan online software. It was found that 3’ UTR of 744 genes mRNA may bind to bta-miR-103, furthermore some target genes were involved in cell proliferation, differentiation and apoptosis. The results of this study laid the foundation for study on the role of bta-miR-103 in the proliferation and differentiation of cattle tissues.

     

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