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产纤溶酶的乳酸菌筛选及其潜在益生特性

Isolation and Probiotic Characteristics of Lactic Acid Bacteria Producing Fibrinolytic Enzyme

  • 摘要: 从传统发酵食品中分离筛选有纤溶活性的乳酸菌并探究其潜在益生功能。以脱脂乳平板法和纤维蛋白平板法筛选出高酶活的菌株,评估其药敏性、抑菌性、胃肠道耐受和黏附性等益生能力。从豆豉中分离到一株产纤溶酶的植物乳杆菌(Lactobacillus plantarum) HQ-3,其发酵上清液纤溶活性为497.6 U/mL,该菌株对13种常见抗生素敏感,对大肠杆菌(Escherichia coli)、金黄色葡萄球菌(Staphylococcus aureus)和沙门氏菌(salmonella) 3种致病菌具有较强抑制作用;经肠胃道模拟试验证明其胃肠道耐受能力强,存活率为72.92%;HQ-3可能具有较好肠道黏附能力,25 h自聚集率为79.6%,与大肠杆菌、金黄色葡萄球菌共聚集率分别为68.9%和57.9%,并且在其基因组中检测到了黏附蛋白基因slp2588。产高活性纤溶酶的植物乳杆菌HQ-3是一株有潜在益生功能的乳酸菌,在食品行业中具有一定的应用价值。

     

    Abstract: The aim of this study was to screen a strain of lactic acid bacteria producing fibrinolytic enzyme from traditional fermented food and explore their potential probiotic functions. The strain of lactic acid bacteria producing fibrinolytic enzyme was screened by using skimmed milk powder plate and fibrinolytic-agarose plate. The potential probiotics of the strain such as antibiotic sensitivity, antimicrobial activity, gastrointestinal tolerance and adhesion ability were evaluated. The strain HQ-3 was isolated from fermented soya beans and the fibrinolytic activity of the supernatant of Lactobacillus plantarum HQ-3 was 497.6 U/m L. HQ-3 was sensitive to 13 common antibiotics and it could inhibit the growth of pathogenic bacteria such as Escherichia coli, Staphylococcus aureus and Salmonella. The survival rate to simulated gastrointestinal tract of HQ-3 was 72.92%. HQ-3 may have good intestinal adhesion ability, after 25 h au-aggregation rate is 79.6%, co-aggregation rates with Escherichia coli and Staphylococcus aureus are 68.9%, 57.9%, the gene of adhesion protein slp2588 was detected. Lactobacillus plantarum HQ-3, which produces highly active fibrinolytic enzyme, is a lactic acid bacteria with potential probiotic function and has certain application value in the food industry.

     

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