Abstract: The aim of this study was to screen a strain of lactic acid bacteria producing fibrinolytic enzyme from traditional fermented food and explore their potential probiotic functions. The strain of lactic acid bacteria producing fibrinolytic enzyme was screened by using skimmed milk powder plate and fibrinolytic-agarose plate. The potential probiotics of the strain such as antibiotic sensitivity, antimicrobial activity, gastrointestinal tolerance and adhesion ability were evaluated. The strain HQ-3 was isolated from fermented soya beans and the fibrinolytic activity of the supernatant of Lactobacillus plantarum HQ-3 was 497.6 U/m L. HQ-3 was sensitive to 13 common antibiotics and it could inhibit the growth of pathogenic bacteria such as Escherichia coli, Staphylococcus aureus and Salmonella. The survival rate to simulated gastrointestinal tract of HQ-3 was 72.92%. HQ-3 may have good intestinal adhesion ability, after 25 h au-aggregation rate is 79.6%, co-aggregation rates with Escherichia coli and Staphylococcus aureus are 68.9%, 57.9%, the gene of adhesion protein slp2588 was detected. Lactobacillus plantarum HQ-3, which produces highly active fibrinolytic enzyme, is a lactic acid bacteria with potential probiotic function and has certain application value in the food industry.