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运输对马肠道菌群的影响

Effect of Transport on Intestinal Microbes in Horses

  • 摘要: 本研究运用Illumina MiSeq测序技术研究长途运输对马肠道菌群的影响,在运输前、运输16 h、运输后16 h 3个时间点采集马肛拭子进行检测。结果显示:运输应激对马肠道菌群多样性和组成有显著影响。Alpha多样性显示马运输后16 h较运输前物种丰富度显著增高(P<0.05)。运输对马肠道菌群在不同分类水平上优势菌群组成分析有不同程度的影响,在门水平上,厚壁菌门(Firmicutes)、拟杆菌门(Bacteroidetes)和变形菌门(Proteobacteria)为三大优势菌门。运输后厚壁菌门、变形菌门平均含量降低,拟杆菌门平均含量升高。在属水平上,链球菌属(Streptococcus)和埃希氏志贺氏菌属(Escherichia-shigella)为两大优势菌属。运输后链球菌属平均含量增长,埃希氏志贺氏菌属平均含量降低。在物种组成差异比较分析中,在门水平上菌群物种组成差异不显著。在属水平上,克里斯滕森氏菌(Christensenellaceae_R-7_group)相较于马运输前的平均含量(2.87%),运输后的平均含量(1.7%)显著降低(P<0.05)。本研究结果显示运输应激会引起马肠道菌群的变化,为进一步研究马运输应激对肠道菌群的影响提供基础,为研究如何缓解马运输应激引起的肠道菌群变化提供参考。

     

    Abstract: The purpose of this study was to investigate the effects of long-distance transportation on the intestinal microbiota of horses using Illumina Mi Seq sequencing technology. In this experiment, anal swabs were collected from horses(Equus) at 3 time points: before transport, 16 h during transport, 16 h after transport. The results showed that transport stress had a significant effect on the diversity and composition of intestinal microbiota in horses.Alpha diversity showed that the species abundance was significantly higher at 16 h after transportation than that before transportation(P<0.05). Transportation has a different degree of influence on the analysis of the composition of the dominant bacteria in the horse intestinal flora at different classification levels. At the phylum level, Firmicutes, Bacteroidetes and Proteobacteria are the dominant phylums. After transportation, the average content of Firmicutes and Proteobacteria decreased, while the average content of Bacteroidetes increased. At the genus level,Streptococcus and Escherichia-shigella are the two dominant bacteria genera. After transportation, the average content of Streptococcus increased while that of Escherichia-shigella decreased. In the comparative analysis of species composition differences, the species composition differences were not significant at the phylum level. At the genus level, the average content of Christensenellaceae_R-7_group after transportation was 1.7%, which was significantly lower than that before transportation(2.87%)(P<0.05). The results of this study show that transportation stress can cause changes in the intestinal microbiota of horses, which can provide a basis for further research on the impact of horse transportation stress on the intestinal microbiota, and provide a reference for studying how to alleviate the changes of intestinal microbiota caused by horse transportation stress.

     

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