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苦瓜多糖和硝酸钠对山羊瘤胃产甲烷古菌多样性的影响

Effects of Momordica charantia polysaccharide and Sodium Nitrate on Diversity of Rumen Methanogens in Goats

  • 摘要: 为研究苦瓜多糖(0, 0.1, 0.3, 0.6 mg/mL)和硝酸钠(0, 5 mmol/L)对体外发酵山羊瘤胃液产甲烷古菌多样性的影响,分别采用末端限制性片段长度多态性分析技术和实时荧光定量PCR技术研究6 h和24 h瘤胃发酵液中产甲烷古菌16S rRNA基因的多样性和产甲烷古菌的数量。结果表明,苦瓜多糖呈二次效应影响6 h发酵液中产甲烷古菌的多样性指数,并对甲烷短杆菌属影响较大,但对24 h发酵液中产甲烷古菌的多样性指数无显著影响。硝酸钠对产甲烷古菌的多样性指数也无显著影响,但显著降低6 h T-RF100的相对丰度。此外,苦瓜多糖显著提高了6 h后产甲烷古菌的数量,并呈二次效应影响24 h发酵液的产甲烷古菌。本研究初步说明添加苦瓜多糖和硝酸钠不仅会对体外山羊瘤胃发酵液中产甲烷古菌的群落造成影响,还会一定程度上影响其多样性,且对产甲烷古菌的群落结构影响具有互作效应。

     

    Abstract: The aim of this research was to study the influence of Momordica charantia polysaccharide(0, 0.1, 0.3,0.6 mg/mL) and sodium nitrate(0, 5 mmol/L) on rumen methanogens of diversity in goats. Terminal restriction fragment length polymorphism(T-RFLP) and quantitative real-time PCR(RT-qPCR) was used to analyse the 16 S rRNA gene diversity and quantities of methanogens on ruminal fermentation at both 6 h and 24 h of in vitro. The results showed Momordica charantia polysaccharide influenced quadraticly methanogenic diversity indexes at 6 h and had a significant effect on Methanobrevibacter ruminantium. However, no significant effects of polysaccharide(at 24 h)and sodium nitrate(at 6 h and 24 h) on methanogenic diversity indexes were observed, but sodium nitrate reduced significantly the relative abundance of T-RF100. In addition, supplementation of polysaccharide increased significantly the quantities of methanogen, and affected quadraticly the quantity of methanogen at 24 h. In conclusion,Momordica charantia polysaccharide and sodium nitrate influenced on the methanogen communities and diversity in vitro rumen fermentation, and had the interactive effects on the methanogen communities.

     

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