鼠伤寒沙门氏菌hilD基因缺失突变株的构建及其表型分析

唐正露; 罗聪玉; 曹堃; 李郁

doi:10.13417/j.gab.040.002748

鼠伤寒沙门氏菌hilD基因缺失突变株的构建及其表型分析

安徽农业大学动物科技学院

基金项目:

国家星火计划重点项目(2014GA710002)

安徽省自然科学基金项目(1508085MC44)

安徽省重点研究与开发计划(面上攻关)项目(201904a06020013)

安徽省长三角联合科技攻关项目(1101c0603065)

安徽省生猪产业体系基金项目[皖农科(2016)84号]共同资助

详细信息

通讯作者:
李郁,liyouer@163.com

中图分类号: S852.61
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出版历程
- 刊出日期: 2021-08-24

Constrution and Phenotypic Analysis of hilD Gene Mutant of Salmonella typhimurium

College of Animal Science and Technology, Anhui Agriculture University

摘要

摘要: 为研究鼠伤寒沙门氏菌(Salmonella typhimurium, S.T) hilD基因的功能，通过同源重组方法构建S.T hilD基因缺失菌株S.T (ΔhilD)，电转化法构建hilD基因回补株S.T (ΔhilD+pcDNA3.1-hilD)、hilD基因缺失株空质粒菌S.T (ΔhilD+pcDNA3.1)和亲本株空质粒菌S.T (pcDNA3.1)，进而对S.T (ΔhilD+pcDNA3.1)与S.T(pcDNA3.1)、S.T (ΔhilD+pcDNA3.1-hilD)的表型进行比较。结果显示，S.T (ΔhilD+pcDNA3.1)和S.T (ΔhilD+pcDNA3.1-hilD)遗传性状稳定；三株菌的生长速率无显著差异(P>0.05);S.T (ΔhilD+pcDNA3.1)的生物膜形成能力显著高于S.T (pcDNA3.1)和S.T (ΔhilD+pcDNA3.1-hilD)的(P<0.05)；而对于小鼠的致病力及对Hela细胞的黏附力和侵袭力，S.T (ΔhilD+pcDNA3.1)均显著低于S.T (pcDNA3.1)和S.T (ΔhilD+pcDNA3.1-hilD)(P<0.05)。表明S.T hilD基因的缺失不影响其正常生长代谢过程，但毒力减弱。
- 鼠伤寒沙门氏菌 /
- hilD基因 /
- 缺失株 /
- 生物学特性
Abstract: In order to study the function of the hilD gene in Salmonella typhimurium（S.T）, the S.T ΔhilD gene deletion mutant S.T（ΔhilD） was constructed by homologous recombination technology, the hilD gene-complementation strain S.T（ΔhilD+pcDNA3.1-hilD）, the hilD gene deletion strain S.T（ΔhilD+pcDNA3.1） and with parental strain S.T with the empty plasmid（pcDNA3.1） were constructed by electroporation, and then the phenotypes of S.T（ΔhilD+pcDNA3.1）, S.T（ΔhilD+pcDNA3.1-hilD） and S.T（pcDNA3.1） were compared. The results showed that the genetic characters of S.T（ΔhilD+pcDNA3.1） and S.T（ΔhilD+pcDNA3.1-hilD） were stable, and the growth rate of the three strains had no significant difference（P＞0.05）. The biofilm formation ability of S.T（ΔhilD+pcDNA-3.1） was significantly higher than that of S.T（ΔhilD+pcDNA3.1-hilD） and S.T（pcDNA3.1）（P＜0.05）. However, the pathogenicity of mice, adhesion and invasion to Hale cells of S.T（ΔhilD+pcDNA3.1） were significantly lower than that of S.T（ΔhilD+pcDNA3.1-hilD） and S.T（pcDNA3.1）（P＜0.05）. In conclusion, the deletion of hilD gene in S.T did not affect the normal growth and metabolism process, but the virulence was reduced.
- Salmonella typhimurium /
- hilD gene /
- Deleted strain /
- Biological characteristics

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